The activation and proliferation of anti-tumor cells are suppressed by Tregs through either contact-dependent or contact-independent mechanisms, where the former inhibits the antigen-presenting capacity of dendritic cells (DC) while the latter secrets various inhibitory cytokines, such as IL-10, IL-35, and tumor growth factor β (TGF-β), to promote an immunosuppressive TME [80,81,82]. This evidence concerns the gene TGFB1 and neoplasm.