ACTA1 and neoplasm: To investigate whether the infiltration of CD8+ and Granzyme B+ cells correlates with Axl expression in tumor cells, we analyzed tissue samples of 40 HCC patients for the infiltration of CD8+ T cells (CD45+ CD8+), Granzyme B+ cells (CD45+ Granzyme B+) and Axl expression in tumor cells (Axl+ CD31- αSMA− CD45−) or immune cells (Axl+ CD45+) by multiplexed immunohistochemistry (Figure 6G).