The International Collaborative of NT-proBNP Study showed that the best predictive values for excluding HF diagnosis in an acute setting were NT-proBNP levels ≥450 pg/mL for ages less than 50, ≥900 pg/mL between 50 and 75 years, and ≥1800 pg/mL for ages greater than 75 years [18]. This evidence concerns the gene NPPB and hydrops fetalis.