In 2019, Liu et al. developed a technique called single-strand library preparation and hybrid-capture-based circulating DNA sequencing (SLHC-seq), which enhances the sensitivity and accuracy of identifying somatic K-ras mutations in pre-cancerous intraductal papillary mucinous neoplasms (IPMNs) and early-stage PDAC, such as stage I and II, by restoring degraded and short ctDNA fragments [78]. This evidence concerns the gene KRAS and pancreatic intraductal papillary-mucinous neoplasm.