Suggesting that Cx32 expression was low in HCC specimens, a significant increase of EGFR, p-EGFR, p-STAT3, and p-Erk1/2 was observed when increasing Cx32 expression, and a significant decrease of EGFR, p-EGFR, p-STAT3, and p-Erk1/2 was observed when downregulating Cx32 expression in HCC cell lines. This evidence concerns the gene GJB1 and hepatocellular carcinoma.