A decreased expression of epithelial marker E-cadherin and an increased expression of the mesenchymal marker Vimentin were observed in the HepG2/DOX cells with downregulated Cx32, while the opposite results were observed in the HepG2/DOX cells with upregulated Cx32 and concomitant decreased invasive and migratory ability, indicating that Cx32 may be responsible for the EMT induction in doxorubicin-resistant HCC cells. The gene discussed is GJB1; the disease is hepatocellular carcinoma.