GJB1 and hepatocellular carcinoma: In their first study, they used tissue samples (76 HCC samples and 20 normal controls), cell lines (normal hepatic cell line: LO2, HCC carcinoma cell lines: HepG2, Huh7, and SMMC-77210), and mouse models (Huh7-hCx32 cells or Huh7-vec cells inoculated) to study Cx32 and EMT markers expression [66].