Towards the same direction, Li et al. investigated the tumorigenic activity of PI3K/Akt signaling in correlation with Cx32 expression, regarding the potential of expanding liver CSCs, using 85 HCC tissue samples, cell lines (HCCLM3 and HepG2), and mouse models (BALB/c) inoculated or not with HCCLM3 (HCCLM3 overexpression—OE HCCLM3 empty vector—EV) [70]. This evidence concerns the gene GJB1 and hepatocellular carcinoma.