Cx43 silencing with a siRNA in SMMC-7221 cell lines resulted in an upregulation of RALA and SRC genes, which transduce signals involved in the control of a variety of cellular processes, including proliferation, differentiation, motility, and adhesion, rendering them novel therapeutic targets in HCC with high Cx43 expression. Here, GJA1 is linked to hepatocellular carcinoma.