Further, as shown by area under the curve (AUC), all groups had significantly (p < 0.05) better glucose clearance compared to mice fed HF, as shown in Figure 3B. For ITT, glucose levels of mice fed HF dropped to 65 mg/dl after insulin injection, followed by increase at 90 min, indicating insulin resistance (Figure 3C). This evidence concerns the gene INS and hydrops fetalis.