By contrast, in spheroids derived from glioblastoma 14-60-4, the Cdkn2a and Mki67 mRNA levels were unchanged, and the transcription rates for Pik3cg, Pten, and Abcb1b increased dramatically, while Sox2 expression decreased, reaching minimal values recorded in the whole experiment. The gene discussed is PTEN; the disease is glioblastoma.