The Cd44lo spheroids showed a weak transcriptomic response to HA exposure, with subtle changes for all 14 genes in spheroids derived from astrocytoma 10-17-2 and oligodendroglioma 51-7 and a 34-fold decrease for Cd44 and Cd133 only in spheroids derived from ependymoma 5. The gene discussed is PROM1; the disease is oligodendroglioma.