Considering that suppression of SRGN expression in GBM cells potently affected the expression of IL-6, IL-8 and TGFβ1 isoform, which are potent signaling molecules that mediate tumor cell–stroma interplay and can regulate the phenotype of stromal cells, we went to investigate the capacity of LN-18shSCR and LN-18shSRGN cells to activate stromal fibroblasts. The gene discussed is TGFB1; the disease is glioblastoma.