Then, we cultured LN-18shSCR and LN-18shSRGN cells, in the absence or in the presence of iTGFβRI and iCXCR-2, to block the autocrine constitutive activation of TGFβRI and CXCR-2 signaling pathways in GBM cells, and culture media were collected in order to study the ability of the above pathways to affect soluble factors produced by GBM cells and influence fibroblasts’ functions. Here, CXCR2 is linked to glioblastoma.