After microarray analysis, the authors selected the four most upregulated circRNAs in BC and their host genes (hsa_circ_0069094 and S100P, hsa_circ_0062558 and MMP11, hsa_circ_0074026 and PITX1, hsa_circ_0079876 and ANLN), and validated them in 121 pairs of BC and adjacent normal tissues by qRT-PCR. This evidence concerns the gene ANLN and breast cancer.