T cells in an activated state change in several respects compared with naive T cells, such as surface protein expression, cytokine secretion, etc. We first detected CD69 (early activation marker) and CD25 (late activation marker) expression of T cells in co-culture (PBMC + drug + NY-ESO-1+ HLA-A*0201+ tumor cell line; E:T = 4:1) after 72 h with FACS. This evidence concerns the gene CD69 and neoplasm.