Furthermore, CBL, LYZ, ISG20L2, PPARA, IGF1R, EIF2AK2, BMP8A, CMTM3, LRP1, etc. were determined as hub genes clustered in the turquoise module, and their upregulation correlated with worse prognosis, suggesting that PAAD patients with high expression of these hub genes were not suitable for this kind of mRNA vaccine. The gene discussed is ISG20L2; the disease is pancreatic adenocarcinoma.