Figure 4 shows the full pathway in greater detail. Mutations affecting PIK3CA upregulate the kinase activity of the PI3K protein, leading to uncontrolled cell growth and proliferation [50]. After determining common PIK3CA-mutated cancer types and prevalent cancer-inducing PIK3CA point mutations, we input these cancers and mutations into AutoEpiCollect. Figure 3B depicts the steps taken by AutoEpiCollect to create a pan-cancer vaccine with this data. Here, PIK3CA is linked to cancer.