POLQ and cancer: While overexpression orloss-of-function of POLQ is useful forlooking at the importance of the protein at a cellular level, studyingthe biochemical kinetics of mutant protein will provide insight intothe mechanism of POLQ in mutagenesis on a molecular level.16 Sporadic and hereditary mutations have beenfound in all five DNA polymerase families expressed in a variety oftumors.17−21 Many of these cancer-associated variants have been characterized in vivo and in vitro to understand theirbiochemical and physiological phenotypes.