This phenomenon was further confirmed by immunohistochemical staining of CD8 and granzyme B (GzmB) (Figs. 5B and EV3), as well as Elisa detection of the multiple inflammatory factors (TNF-α, IFN-γ, IL-2, IL-6, IL-12) in Tumor1 and Tumor2 of HCC tumor-bearing mice with Poly(I:C) plus RT treatment (Fig. 5C). The gene discussed is TNF; the disease is hepatocellular carcinoma.