To clarify the role of poly(I:C)-activated intratumoral infiltrating CD8+ T cells in abscopal effect, CD8+ T cells derived from Tumor2 of bilateral HCC subcutaneous tumor models constructed in C57BL/6 mice or TLR3-knockout mice were both stimulated with PBS or poly(I:C), respectively, and further co-cultured with non-irradiated Hepa1-6 cells to mimic the recruitment of CD8+ T cells into the non-irradiated distant tumor (Fig. 6A). This evidence concerns the gene TLR3 and hepatocellular carcinoma.