These results verified that poly(I:C)-activated intratumoral CD8+ T cells, but not poly(I:C) itself, promoted the ferroptosis-related lipid peroxidation damage and tumor killing in non-irradiated Hepa1-6 cells, suggesting the key role of intratumoral infiltrating CD8+ T cells in poly(I:C) promoted distant tumor ferroptosis and abscopal effect depending on the activation of TLR3 signaling. The gene discussed is CD8A; the disease is neoplasm.