ATM and acute myeloid leukemia: We then isolated the leukaemic stem cells (LSCs, YFP+/ c-kit+) from established AML mice (F0 recipients) and employed in vivo limiting dilution transplantation assays to assess the capacity of LSCs to be affected by Atm loss; 1,000/500/100 LSCs from the former recipients were used to generate the next generations (Supplementary Fig. S1E).