While the PMA-Ionomycin-treated groups exhibited consistent outcomes across all experimental conditions (Supplementary Fig. 7b, c), PD-1 KO enhanced the frequency of CAR-T cells concurrently releasing IFN-γ and TNF-α during co-culture with HER2+ tumor cells in the context of LA CAR-T cells. The gene discussed is TNF; the disease is neoplasm.