We found that AIE produced anxiety-like behavior (reduction in % time spent in light box) in both male (Two-way ANOVA, group x siRNA infusion interaction; F1,28 = 12.14, p < 0.01) (Fig. 2D) and female (Two-way ANOVA, group x siRNA infusion interaction; F1,23 = 12.37, p < 0.01) (Fig. 2E) rats, and was attenuated by the EZH2 siRNA infusion into the CeA in both sexes (Fig. 2D, E). The gene discussed is EZH2; the disease is Anxiety.