BNIP3 and non-small cell lung carcinoma: When cells of the human non-small cell lung carcinoma H1299 line were treated under hypoxia (1% O2), HIF1α protein was stabilized (Supplementary Fig. 1a), and the typical hypoxia-responsive genes, including PDK1, GLUT1, LDHA, VEGF and BNIP3, were largely upregulated46, indicating that the hypoxic condition was achieved (Supplementary Fig. 1b).