Finally, global assessment considering Sirius Red/Fast Green and Col IV staining, amongst others (Table S2), confirmed that 3-mo-old Clcn5+/− and Clcn5−/− mice already present higher levels of renal fibrosis than Clcn5+/+ mice (no fibrosis: 100% versus 33.3%, fibrosis degree >1: 66.6% versus 0%, P < 0.01) (Fig 7E). The gene discussed is CLCN5; the disease is fibrosis.