In addition, we observed a significant reduction in gene expression of Nrf2 targets and a concomitant decrease in HCC markers Mat2a (methionine adenosyltransferase 2A), Afp, Gpc3 (glypican 3), c-Myc in Cas9-treated mice compared to NTBC-off–diseased mice (Supplemental Figure S14, http://links.lww.com/HC9/A847). This evidence concerns the gene GPC3 and hepatocellular carcinoma.