Similar to the PFAS-mediated changes in mouse testes, there were a consistent downregulation of gene sets associated with the H3K27me3 mediated polycomb pathway and an alteration in gene sets associated with DNA methylation, as well as alterations in cancer pathways associated with tumorigenesis, apoptosis, hypoxia, and DNA repair (Figure 5 and Supplemental Table S2). This evidence concerns the gene PFAS and cancer.