Indeed, as expected, not only all these three MARCH8 proteins inhibited both VSV-G and HIV-1 Env-mediated infection, but also their highly conserved RING-CH domains or tyrosine motifs were found to be antiviral determinants against either both VSV-G and HIV-1 Env or only HIV-1 Env, respectively. This evidence concerns the gene ERVW-1 and infection.