ERVW-1 and infection: Our findings revealed that rhesus, mouse, and bovine MARCH8 proteins harboring the W114A mutation exhibited a partial loss of inhibitory activity against both VSV-G- and HIV-1 Env-mediated infection (Figure 1C,D), whereas all the AxxL mutants lost inhibitory activity against only HIV-1, consistent with observations from human MARCH8 mutants (Figure 1D).