GAPDH and cancer: Figure 1A shows a decrease in the DCN mRNA level in all CAFs, as compared to their adjacent TCFs (100%). To confirm this at the protein level in cancer-associated fibroblasts, whole cell extracts were prepared from the same TCF/CAF pairs, and specific anti-DCN and anti-GAPDH (used as internal control) antibodies were utilized for immunoblotting analysis. Figure 1B shows that the level of the DCN protein was lower in all CAFs (100%) as compared to their corresponding TCFs.