PTEN and neoplasm: In corroboration with PTEN loss, activation of AKT signalling in CU-PC01 PDX tumours was confirmed by Western Blotting for p-AKT (T308) and IHC to detect AKT downstream substrates p-4EBP1 (T37/46) and p-RPS6 (S235/236) in CU-PC01 tumours (Supplementary Figure S3).