In silico analysis was performed to address this point, with RAN and MAPK3 as the genes that were significantly co-expressed with PRMT5 in HCC patients, the genes that correlated with worse prognosis, and genes that were downregulated upon PRMT5 depletion were shortlisted (Supplementary Figure S2). The gene discussed is MAPK3; the disease is hepatocellular carcinoma.