Similar to transgenic Tg-BBLN mouse hearts, cardiac specimens of human TOF patients with increased BBLN contents and cyanosis also showed increased phospho-T287–CAMK2D contents compared with those of acyanotic TOF patients, whereas total CAMK2D protein levels were not significantly different between the two groups (Extended Data Fig. 9a). This evidence concerns the gene CAMK2D and Cyanosis.