One cycle of only GEM, IV route, 1,000 mg/m2 on 1st, 2nd, and 15th days in a 4-week cycle, followed by GEM+Vaccine. GEM dosing and timing are similar to the first cycle. The vaccine is given via the ID route twice a week at six different sites. The initial treatment protocol was planned as three courses. 2 PDA patients and 1 ICC patient were given WT1/DC/I. 1 patient with PDA was treated with WT1/DC/II. The remaining 7 patients with PDA were given WT1/DC/I+II. The gene discussed is WT1; the disease is Patent ductus arteriosus.