Previous studies have demonstrated that celastrol can directly inhibit the kinase activities of PI3K[12] and IKKβ.[21] Moreover, multiple studies have associated the impact of celastrol on the PI3K/Akt and NF‐κB signaling pathways with its anti‐tumor and anti‐inflammatory activities.[21, 22] Therefore, we attempted to establish a target validation system using these two known targets. The gene discussed is AKT1; the disease is neoplasm.