SUN1 and amyotrophic lateral sclerosis: Based on these results, we decided to evaluate changes to the nuclear morphology (i.e., area and circularity) and soma size in cortical neuron divided into four sub-groups: (1) Control neurons, used as a reference (2), ALS neurons with normal SUN1 and TDP-43 staining (3), ALS neurons with disrupted SUN1 staining but normal TDP-43 distribution, and (4) ALS neurons with disrupted SUN1 staining and abnormal cytoplasmic TDP43 mislocalization.