When we performed a similar analysis in the spinal cord samples, we again observed in both sALS and C9-ALS motor neurons a significant reduction in the absolute nucleolar area (13.99 ± 4.75 μm for sALS, 9.73 ± 3.93 μm for C9-ALS and 22.71 ± 4.77 μm for CTRL) and a trend toward smaller nuclei (166.8 ± 40.80 μm for sALS, 156 ± 51.36 μm for C9-ALS and 204.5 ± 40.61 μm for CTRL) compared to non-neurological controls (Supplementary Fig. 12d-f). This evidence concerns the gene C9 and amyotrophic lateral sclerosis.