To investigate the potential role of NCEH1 in diabetes-induced endothelial dysfunction, we examined the activity of NCEH1 and the expression levels of NCEH1 in HG-incubated mouse aortae ex vivo, HG-induced endothelial cells in vitro, or the aortae from HFD-induced obese mice in vivo. This evidence concerns the gene NCEH1 and diabetes mellitus.