TGFB1 and neoplasm: On the other hand, even though MCF10A cells often skip NEDD4Le13 in untreated conditions, they did not show a response to SSO-0205 under TGFβ treatment, perhaps due to differences in NEDD4Le13 regulation between the cell types and a dependency on the short isoform in the context of tumor maintenance (Fig. 7A).