Using MDA-MB-231 as a representative TNBC cell line, and MCF10A (cell line derived from breast fibroadenoma) as a control, we evaluated the splicing patterns of NEDD4Le13 in the context of TGFβ stimulation via treatment with human recombinant TGFβ (hrTGFβ). The gene discussed is TGFB1; the disease is Fibroadenoma.