The results showed that the top 10 high mutation frequency genes in HCC patients were TP53 (28%), TTN (25%), CTNNB1 (24%), MUC16 (16%), PCLO (11%), ALB (11%), MUC4 (10%), ABCA13 (9%), RYR2 (9%), and APOB (9%) (Fig. 2B). The gene discussed is RYR2; the disease is hepatocellular carcinoma.