Instead, E10 of CERS5 is alternatively spliced between GBM and normal brain tissue and the PSI of CERS5-E10 is significantly correlated with C16-ceramide abundance (Figure 6D and Supplemental Figure 5C), indicating an isoform-specific function of CERS5 in increasing the levels of C16-ceramide in GBM. This evidence concerns the gene CERS5 and glioblastoma.