In contrast, microglia from GVHD mice exhibited increased expression of major histocompatibility complex class I and II MHC genes (i.e., H2-K1, B2m, CD74, and H2-Ab1), as well as chemokine genes (i.e., Ccl2, Ccl3, Ccl4, Ccl5, Ccl7, Ccl12, Cxcl9, and Cxcl10), indicative of an activated inflammatory phenotype (Figure 2E). This evidence concerns the gene CCL4 and graft versus host disease.