Using this approach, we observed that microglia from GVHD animals had enriched regulon activity for Stat (i.e., Stat1, Stat2, Stat3, Stat4, and Stat6), NF-κB (i.e., Nfkb1, Rela, and Rel), and IFN regulatory factor (i.e., Irf1, Irf2, Irf3, Irf7, and Irf9) family transcription factors (Figure 2G), which are all constituents of regulatory networks that mediate inflammation (37). Here, STAT3 is linked to graft versus host disease.