GOLIM4 and lung cancer: To identify GOLIM4-dependent secreted mediators, we performed liquid chromatography–mass spectrometry (LC-MS) analysis on CM samples from 3q-amplified human lung cancer cells that were GOLIM4 deficient or replete and identified a total of 308 proteins, 64 of which were downregulated by GOLIM4 depletion (P < 0.05, fold change >1.5; Figure 3F and Supplemental Table 1).