Compared with normal hematopoietic cells, AML-related genes have been shown to expressed at higher levels in eccDNA during the development of AML, including NRAS, MCL1, EVI1, GATA2, WT1, PAK1, and GLYATL1. Therefore, during the development of AML, the accumulation of eccDNA and oncogenes (NRAS, MCL1, EVI1, GATA2, WT1, PAK1, and GLYATL1) in eccDNA may contribute to the progression of AML. The gene discussed is PAK1; the disease is acute myeloid leukemia.