To verify the classification effect of TOP2A expression on the samples with colitis and healthy controls, the original sample data of GSE9452, and GSE53306 was used to draw the ROC curves of NDC80, PBK, CEP55, RRM2, ASPM, NCAPG, TOP2A and CDKN3 by R language (Figure S4). This evidence concerns the gene PBK and colitis.