PAK1 and breast cancer: We inoculated human breast cancer TMX-28 cells (a variant of the MCF-7 breast cancer cells(Fasco, Amin, Pentecost, Yang, & Gierthy, 2003), stably overexpressing GFP, PAK1 WT or PAK1 Y3F (the clones were characterized previously in(Rider et al., 2013)) into the mammary pads of NSG mice.