We inoculated human breast cancer TMX-28 cells (a variant of the MCF-7 breast cancer cells(Fasco, Amin, Pentecost, Yang, & Gierthy, 2003), stably overexpressing GFP, PAK1 WT or PAK1 Y3F (the clones were characterized previously in(Rider et al., 2013)) into the mammary pads of NSG mice. This evidence concerns the gene PAK1 and breast carcinoma.