To determine whether local production of DKK1 at tumor site limits the infiltration of immune populations, we performed IHC to determine the localization of CD45+ cells in PyMT tumors isolated from IgG or mDKN01 treated mice (Fig. 5B), aSMA-Dkk1WT or aSMA-Dkk1cKO animals (Fig. 5C) and from WT mice co-injected with tumor cells together with aSMA-Dkk1WT or aSMA-Dkk1cKO CAFs (Fig. 5D). The gene discussed is DKK1; the disease is neoplasm.