DUOX2 and colitis: AOM/DSS Chemically induced model that replicates the aspect of colitis associated tumorigenesis is widely used.40,41 The knockout of Duox2 gene in mice causes both hypotension and bradycardia basally.39 To exclude the possibility that these deficiencies confound our findings, we generated intestine-specific Duox2-deficient mice by crossing Duox2fl/fl mice with Villin-Cre transgenic mice, which specifically deleted Duox2 from intestinal epithelia (denoted as Duox2 CKO mice) to investigate the effect of Duox2 deficiency on composition of GM (genotype by PCR shown in Supplemental Figure S5).