Moreover, network construction produced similar results: Rumonococcus, Lachnospiraceae_NK4A136-group, Prevotellaceae_NK3B31_group, and Clostridium_sensu_stricto_1 were involved in the accumulation of IL-1β, TNF-α, MG,AGEs, TG, AgRP, and SOCS3 for the development of obesity (Figure 12B). The gene discussed is TNF; the disease is obesity due to melanocortin 4 receptor deficiency.