The CCK8, plate cloning, EdU cell proliferation, wound healing, transwell, and WB assays showed that PIK3IP1 knockdown reversed the effects of circPDE5A overexpression on the proliferation, motility, migration, invasion, and EMT of ESCC cells (Fig. S4A-B, and E–H), while PIK3IP1 overexpression reversed the effects of circPDE5A knockdown on the proliferation, motility, migration, invasion and EMT of ESCC cells (Fig. S4C-D, and I-L). The gene discussed is PIK3IP1; the disease is esophageal squamous cell carcinoma.