PDE5A and esophageal squamous cell carcinoma: The CCK8, plate cloning, EdU cell proliferation, wound healing, transwell, and WB assays showed that transfection of p-circPDE5A and p-PDE5A-500aa vectors inhibited the proliferation, motility, migration, invasion, and EMT of ESCC cells, whereas transfection of p-circPDE5A-Mut reversed the effects caused by transfection with circPDE5A and PDE5A-500aa (Fig. 5A–F).