In an attempt to further expand our understanding of the relationship between PD-L1 expression and cancer aggressiveness, we investigated PD-L1 expression by immunohistochemistry (IHC) in a large cohort of 2710 bladder cancer patients including 512 patients with follow-up data who underwent radical cystectomy and follow-up therapies in the pre-CPI era. This evidence concerns the gene CD274 and urinary bladder carcinoma.