Interestingly, we also observed in parental PC-9 cells that 24 hours of osimertinib treatment was associated with increased nuclear localisation of YAP1/WWTR1 (with no change in total expression levels, Supplementary Fig. 4e); suggesting that modulation of Hippo signalling is an acute response to EGFR inhibition in EGFR mutant lung cancer and might cause drug resistance (Fig. 2e). This evidence concerns the gene WWTR1 and lung cancer.