In this study, we analyzed the situation of TMB in patients with Adenocarcinoma of the lung, screened out the gene modules most related to TMB through WGCNA analysis, and conducted enrichment analysis to find that most genes in the modules are closely related to cell cycle, DNA repair, replication, etc. In addition, based on univariate regression analysis, LASSO analysis, and multivariate regression analysis, a dual gene model (ANLN and E2F7) with good prognosis prediction ability was constructed. The gene discussed is E2F7; the disease is lung adenocarcinoma.