Additionally, a team observed a similar increase in NETs and neutrophils in a mouse model of transient middle cerebral artery occlusion (tMCAO) and noted a reduction in HMGB1 and NETs, an alleviation of brain damage and improved neurological function after platelet depletion; the opposite results were observed when recombinant HMGB1 was administered. This evidence concerns the gene HMGB1 and medical procedure.