Limitation of our study include lack of the SHBG measurement and, therefore calculated free serum testosterone which may have underestimated the prevalence of hypogonadism in HIV patients since measurement of SHBG has been highly recommended in men with HIV in addition to serum LH and TT due to the possible rise in serum SHBG in these patients [56–58]. The gene discussed is PLOD1; the disease is hypogonadism.