This study established an in vitro model of the intestinal mucosal epithelial model in sepsis using LPS stimulation to induce human colorectal adenocarcinoma cells (Caco−2) to investigate whether the mTOR/P70S6K signalling pathway regulates HIF−1α expression to attenuate sepsis-induced impairment of intestinal mucosal epithelial model function. The gene discussed is HIF1A; the disease is Sepsis.