Intracellular fat accumulation was evaluated in the FFA + si-TCONS-00039830, FFA + miR-455-3p mimic and FFA + TCONS-00039830 + miR-455-3p mimic groups to examine the role of TCONS-00039830/miR-455-3p/Smad2 in hepatic steatosis. Here, SMAD2 is linked to fatty liver disease.