PICRUSt analyses outlined 65, 43 and 74 functions altered in fecal samples from mice with end point TRAMP-C2 tumours, Pten−/−; Rb1+/+ and Pten−/−; Rb1−/− tumours compared to their corresponding no tumour controls, respectively (Fig. 4e, Fig. S2A–E). The gene discussed is PTEN; the disease is neoplasm.