We also noted 0.7 ± 0.2% and 0.5 ± 0.2% Ruminococcaceae_g_2 increase in both Pten−/−; Rb−/− (Fig. 4b) and Pten−/−; Rb1+/+ fecal samples compared to no tumour controls (Fig. S1B). Here, RB1 is linked to neoplasm.